In this Application Note, the optimization of the filtration process of a mAb-contained CHO cell culture is shown in detail. So far, the suspension is filtered in three steps, before a sterile filtration using a 0.2 μm membrane is applicable. To ensure the sterile filtration step, a flocculant has to be added prior to each filtration. In order to reduce the number of process steps in the cell harvest process, filtration tests with FILTROX depth filter sheets are performed in the laboratory scale.

The aim is to determine the most suitable filter aid and its ideal quantity and, in addition, to achieve turbidity values below 30 NTU to ensure subsequent sterile filtration. 

READ THE APPLICATION NOTE

Save one process step The main advantage of alluvial filtration for the clarification step of Plasmid-DNA is the elimination of the two-step approach. Due to the increased filtration capacity the traditional approaches such as centrifugation followed by depth filtration or a two-step depth filtration are not necessary anymore. The simultaneous reduction of impurities e.g. HCP is another advantage.

Find out more on our PURADISC® BIO SD webpage or contact our filtration experts:

Read the new Paper of the Royal Society of Chemistry about a novel instrumental analytical endotoxin quantification assay.

Download the Paper

Find also more information on the rsc.org page.

The process combines low running costs with operating robustness. 

Find out more on our webpage or contact our filtration experts:

LINKS AND DOWNLOADS:

LEAFLET MAMMALIAN CELLS AND MAb CLARIFICATION 

MORE ABOUT THE CLARIFICATION SOLUTIONS FOR MAMMALIAN CELLS AND MAb’s

Linear scale up
from lab to production.

Reduction of costs and production steps
by saving the centrifuge step.

Single-use solution 
eliminates cross-contamination risks and reduces cleaning validation efforts.

Find out more on our webpage or contact our filtration experts:

LINKS AND DOWNLOADS:

VIDEO FILTRODISC BIO SD 

ABOUT ALLUVIAL FILTRATION (PDF download)

BROCHURE CARBOFIL™ (PDF download)

VIDEO CARBOFIL™ WITH ACTIVATED CARBON

GO TO THE TECHNICAL DATA SHEETS

FILTRATION PRACTICE

From laboratory to production scale. How to find the optimal solution for your filtration process. From laboratory trials to estimate the perfect filter sheet to calculation for your individual production scale. Read the application note: https://bit.ly/2XS4leh

An Economical Solution for Midstream Clarification

Introduction
Continuous process optimization is a key factor in the biotech industry. With higher particle loads (>108 cells/ml), standard technologies for cell removal (midstream processing) – e.g. centrifugation, separation, membrane- and depth filtration – find their limits.

To increase efficiency and making the midstream process more economical alluvial filtration is the state-of-the-art solution. Alluvial filtration is a type of depth filtration and a well-established, econo-mical method in pharmaceutical industries (e.g. plasma fractionation). Instead of using an immobilized depth filter medium, filter aid (e.g. diatomaceous earth, perlite) is used to constantly build a filter cake during filtration. The filter cake with its resistance acts then as the actual filter medium. Alluvial filtration, therefore, leads to a higher filter capacity – especially with compressible particles, e.g. microbial or mammalian cells. This technology leads to a maximum product yield and highest economic efficiency and is linear scalable from lab to production scale.

Read more (PDF)

Producing pure and safe CBD products is the goal of every quality-conscious manufacturer of cannabis products. Raw hemp extracts contain high concentrations of cannabinoids, but will also contain contaminants and impurities like plant particles, chlorophyll and debris as well as unwanted oils and waxes. Why certain contaminants should be removed:

Chlorophyll:
• bitter taste and highly unstable
• will cause degradation of cannabinoids

 Waxes:
• very sticky components
• removal leads to smoother texture and consistency

Filtration and purification steps of CBD products and oils

In order to ensure product quality and safety, filtration is essential. To remove the waxes, fats and oil from the winterization a multi-step filtration is recommended. Depending on the batch size different approaches are possible. Find out more about the filtration steps. more…

Would you like to get to know us personally and find out more about our depth filtration solutions? Visit us at MJBizCon in Las Vegas, Dec 11-13, 2019 / Stand C9238.  MJBizCon, is the largest gathering of cannabis business professionals in the world. With 35,000 attendees and programming to benefit every sector, there is no place like it to equip your business for growth. We look forward to meeting you there. more about MJBizCon

How optimization of the filtration step saves time and reduces operating costs. Download the application note.

There are several ways to extract the CBD rich oil for therapeutic use from the dried and heated buds. The most common extraction methods are ethanol and CO₂ extraction. To find out more about the cannabis oil filtration possibilities.

 download the White Paper.